HACCPLAN

INOCULATION / SPAWNING

Cultivation & Harvest

Inoculation / Spawning

Prerequisite

Also: spawn-run · seeding · vinegar mother culture

Introducing desired culture (mushroom mycelium, fermentation starter, sprout seed) into prepared substrate.

Typical Hazards

B
Biological
Contamination by competing pathogens (Aspergillus, E. coli on sprout seed)
C
Chemical
Allergen carryover via shared spawn equipment
P
Physical
Spawn-grain fragments

Typical Controls

Validated seed/spawn treatment (e.g. 20,000 ppm Ca(ClO)2 sprout seed soak)
Sanitized environment for inoculation room (positive pressure, HEPA where possible)
Documented spawn source and lot

Regulatory Citations

2 cross-references across 1 documents

21 CFR Part 112